Please use this identifier to cite or link to this item: http://202.45.146.37:8080/jspui/handle/123456789/126
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dc.contributor.authorRai, Januka
dc.date.accessioned2019-09-02T05:40:11Z
dc.date.accessioned2020-12-21T07:51:55Z-
dc.date.available2019-09-02T05:40:11Z
dc.date.available2020-12-21T07:51:55Z-
dc.date.issued2019-09-01
dc.identifier.urihttp://202.45.146.37:8080/jspui/handle/123456789/126-
dc.descriptionA Dissertation Submitted to the Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, Nepal, in Partial Fulfillment of the Requirements for the Award of Degree Master of Science in Microbiology (Agriculture Microbiologyen_US
dc.description.abstractPseudomonas fluorescens is Plant Growth Promoting Rhizobacteria that aggressively colonize the root zone and promote plant growth are generally termed as Plant Growth Promoting Rhizobacteria (PGPR). P. fluorescens act as biocontrol agents as well as plant growth promoter as it produce different types of secondary metabolites likes iron chelating siderophores, hydrogen cynide, exoenzymes, phytohermones, antibiotic and helps to adopts the plants from various stressed conditions. They also protect plants from phytopathogens by controlling or inhibating them, improves soil structure, bioremediates that pollutes soil by sequestering toxic heavy metal species and degrading xenobiotic compounds like pesticides. The aim of study is to isolate and identify P. fluorescens from rhizospheric soil of Dhankuta, Sunsari, Morang, Jhapa and Illam to observe its effects on growth of medicinal plants and its biocontrol effect on some phytopathogens. The rhizospheric soil was collected from 5 districts of eastern Nepal, in sterile plastic bag and cultured in king’s B media by serial dilution and incubated at 25°C for 48 hrs. Morphological, cultural, biochemical and physiological characterization was done and bacteria were identified. Isolated P. fluorescens were cultured on Kings B broth and inoculated on medicinal plants by deep root technique. In vitro effects on selected phytopathogens were observed on Modified Kings B media by dual culture method. It was found that marigold and mint inoculated with P. fluorescens was so healthy with increase in height, no. of branches, fresh and dry weight. Increament in height of marigold and mint was found to be around 31% and 28.9% comparing to that of uninoculated plants. Similarly, different isolates were found to suppress different plant pathogens being the D1 most potent in suppressing S. rolfsii, JF and DR being most potent in suppressing R. solani and Alternaria spp respectively. While other isolates were found to moderate potent.en_US
dc.description.sponsorshipA Dissertation Submitted to the Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, Nepal, in Partial Fulfillment of the Requirements for the Award of Degree Master of Science in Microbiology (Agriculture Microbiologyen_US
dc.language.isoenen_US
dc.publisherCentral Campus of Technology Institute of Science and Technology Tribhuvan University, Nepalen_US
dc.subjectPGPRen_US
dc.subjectmedicinal plantsen_US
dc.subjectphytohermonesen_US
dc.subjectsiderophoreen_US
dc.titleEFFECT OF Pseudomonas fluorescens ON GROWTH OF MEDICINAL PLANTS AND ITS BIOCONTROL EEFECT ON SELECTED PHYTOPATHOGENSen_US
dc.typeThesisen_US
Appears in Collections:Thesis

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