Please use this identifier to cite or link to this item: http://202.45.146.37:8080/jspui/handle/123456789/139
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dc.contributor.authorShrestha, Romika
dc.date.accessioned2019-09-10T02:10:49Z
dc.date.accessioned2020-12-21T07:51:53Z-
dc.date.available2019-09-10T02:10:49Z
dc.date.available2020-12-21T07:51:53Z-
dc.date.issued2019-09-10
dc.identifier.urihttp://202.45.146.37:8080/jspui/handle/123456789/139-
dc.descriptionA Dissertation Submitted to the Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, Nepal, in Partial Fulfillment of the Requirements for the Award of Degree of Master of Science in Microbiology (Agriculture)en_US
dc.description.abstractFusarium wilt acts as a limiting factor for the yield of tomato for which Trichoderma spp. has been evidently used as a biological control agent against the wilt. However, the efficiency of fungi is not well understood. The main purpose of this study was to investigate Trichoderma harzianum isolates towards their contribution to growth of tomato in Eastern Nepal to counteract the effect caused by Fusarium wilt. Investigation of T. harzianum was performed under in vitro and in vivo conditions against the pathogen (Fusarium oxysporum). The most dominant species and causative agent of Fusarium wilt was identified as Fusarium oxysporum. Three native Trichoderma antagonists were isolated from fifteen soil samples of different geographical regions of Eastern Nepal. Under in vitro conditions, the results revealed that Trichoderma harzianum, isolate Th-TJ, was found to inhibit effectively the radial mycelial growth of the pathogen by (57%). Under greenhouse conditions, the application of T. harzianum, Th-TJ exhibited the least disease incidence. Also, tomato plants treated with T. harzianum, Th-TJ isolate showed a significant stimulatory effect on plant height by (78.33 cm) and the dry weight by (3.33 g) of tomato plants, in comparison to untreated control (1.4 g). Therefore, the antagonist T. harzianum, Th-TJ is chosen to be the most promising bio-control agent for F. oxysporum f.sp. lycopersici. On the base of this study, the biocontrol agents of plant diseases might be exploited for sustainable disease management programs to save environmental risk.en_US
dc.description.sponsorshipCentral Campus of Technology, Tribhuvan University, Dharan, Nepal, in Partial Fulfillment of the Requirements for the Award of Degree of Master of Science in Microbiologyen_US
dc.language.isoenen_US
dc.publisherDepartment of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, Nepal, in Partial Fulfillment of the Requirements for the Award of Degree of Master of Science in Microbiologyen_US
dc.subjectBiological controlen_US
dc.subjectFungi antagonisten_US
dc.subjectFusarium wilten_US
dc.subjectLycopersicon esculentumen_US
dc.titleEVALUATION OF Trichoderma harzianum AS A BIOCONTROL AGENT ON Fusarium WILT OF TOMATO GROWN IN EASTERN NEPALen_US
dc.typeThesisen_US
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