FRUITS PEEL WASTE AS A FUNGAL GROWTH MEDIUM A

Abstract

Most of the fruits peels are either dumped or incinerated in large extent once after the main part of fruits are used. There is an urgency to mitigate this sort of action, because at foremost, these fruits and vegetable peels are more useful for either agricultural purpose or for other different research activities in the coming future. The unused fruit peels can be simply used in different microbiological media preparing industries and in the pharmaceutical industries to some extent. The present study was aimed to formulate growth media using fruits peel waste materials such as Pineapple, Papaya, Green Banana, Yellow Banana, Orange and Pomegranate. The collected peels were air dried, grinded into fine particles using mechanical blender. Fungi isolates (Aspergillus niger and Rhizopus stolonifer) were isolated from soil and air (by serial dilution and open plate technique) using potato dextrose agar (PDA) and identified it. Similarly, Saccharomyces cerevisiae was isolated from Marcha sample. About 4.0 grams of dried fruits peel powder were added into the 100 ml of distilled water and sterilized by autoclaving. After Sterilization and cooling, one ml of fungal inoculums was added and incubated at room temperature for 5 days. The fungi growth was visually observed. Aspergillus niger growth was recorded in the medium containing Pineapple, Orange, Green banana, Yellow Banana and Pomegranate except Papaya. Rhizopus stolonifer growth was observed in the medium which contains Pineapple, Green Banana, Yellow Banana and Pomegranate except Papaya and Orange. However, the Saccharomyces cerevisiae growth was recorded in the medium which contains Pineapple, Green Banana, Orange and Papaya only except Yellow banana and Pomegranate.