ANTIBIOGRAM OF MERCURY RESISTANT Pseudomonas aeruginosa FROM SEWAGE SAMPLES OF EASTERN, NEPAL

Abstract

Sewage is the combination of wastewater from sources and it consists of all sorts of nutrient for microbial growth. It provides shelter for many microorganisms like Salmonella, Shigella, Escherichia coli, Streptococcus, Pseudomonas aeruginosa, Mycobacterium, Giardia lamblia. It may also contain heavy metals. Sometimes sewage contains mercury and its compounds which make the organisms mercury resistant. Pseudomonas aeruginosa present in sewage may develop mercury resistance by certain morphological changes or by genetic process. The main objective of this study is to determine the antibiogram of P. aeruginosa. In this study, 50 samples were collected from different areas of Sunsari and Morang by using BOD bottles and they were diluted by 10 folds and spread over the Cetrimide agar containing different concentration of mercuric chloride and minimum inhibitory concentration for mercuric chloride was determined. Antibiogram was performed against 13 different recommended antibiotics. P. aeruginosa isolates were found resistant to amoxicillin, ampicillin, clindamycin, nalixidic acid and vancomycin and also complete intermediate resistance was shown towards erythromycin. P aeruginosa showed complete susceptibility towards tetracyline. Whereas other P. aeruginosa isolates were more or less intermediately resistant to rest of the antibiotics.