ANTIBIOGRAM OF BACTERIAL ISOLATES CAUSING OTITIS MEDIA FROM OTITIS MEDIA SYMPTOMATIC PERSON’S OF DHARAN

Abstract

Otitis media is a common problem to all age group especially to the children, which causes inflammation of middle ear. This study aimed to isolate and identify the bacteria responsible for otitis media and perform the AST profile of identified isolates, from people having some sort of ear problems. This is a laboratory based cross- sectional study. The ear swab sample was taken from students of different schools, students of Central Campus of Technology, workers of Central Campus of Technology, complaining of ear problems. The samples taken were ear swab in the sterile cotton swab and all the collected samples were taken to the Laboratory and were processed as soon as possible in the Laboratory of Department of Microbiology. The total sample collected was 50. Out of 50 samples, 18 were from children, 16 were from adults and 16 were from old people. The 26 samples were form male and 24 samples were from female. Out of total, 92% showed the growth of bacteria and 8% did not showed growth. The bacteria isolated were Staphylococcus aureus (26%), Staphylococcus epidermidis (38%), Bacillus spp. (18%), Pseudomonas aeruginosa (6%), Haemophilus spp (2%). and Moraxella spp (2%) respectively. The highest number of isolates was seen in the children of age5- 10. The least number of isolates was seen in the age group (21-50). The antibiotics used were Ciprofloxacin, Azithromycin, Gentamicin, Ceftazidime, Cotriomoxazole, Chloramphenicol, Imipenem and Ampicillin. Ciprofloxacin and Gentamicin were most effective which showed 100 % sensitivity. The least effective antibiotic was Ampicillin which showed 100 % resistivity.