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http://202.45.146.37:8080/jspui/handle/123456789/28| Title: | EXTRACTION OF AMYLASE FROM Bacillus subtilis ISOLATED FROM SOIL OF DHARAN, SUNSARI |
| Authors: | Maskey, Chetan |
| Keywords: | Amylase Characterization Extraction Soil Starch Hydrolysis |
| Issue Date: | 28-Nov-2018 |
| Abstract: | Amylases are the enzymes that catalyzes the hydrolysis of starch, glycogen, and related polysaccharides to oligosaccharides, maltose, or glucose. In present study, amylase producing bacteria were isolated from five soil samples of different places of Dharan. A total of 25 bacterial colonies were isolated from collected soil samples. Five bacterial isolates, exhibited zone of clearance in starch hydrolysis test. The isolated displaying maximum amylase activity on quantitation were selected characteristics features of strain indicates that it belongs to the genus Bacillus will be later used for further characterization and amylase extraction. Maximum yield of amylase was obtained after 48hours incubation. The optimum pH for enzyme activity was found to be at pH 7.0 and the optimum temperature for the activity was found to be 40oC. |
| Description: | A Project Work Submitted to Department of Microbiology Central Campus of Technology, Tribhuvan University In Partial Fulfillment for the Award of the Degree of Bachelor of Science in Microbiology Submitted by Chetan Maskey Roll Number: 80015 Reg. Number: 5-2-0008-0029-2013 © Tribhuvan University August, 2017 |
| URI: | http://202.45.146.37:8080/jspui/handle/123456789/28 |
| Appears in Collections: | B.Sc. Microbiology |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Chetan Maskey.pdf | 1.14 MB | Adobe PDF |  View/Open |
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