PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ANALYSIS OF Tectaria coadunata IN DHARAN, EASTERN NEPAL

Abstract

This study aims to evaluate phytochemicals and antimicrobial activity of medicinal ferns Tectaria coadunata. It was conducted from September 2022 to April 2023. Ferns which have been commonly used by local people for medicinal purpose Tectaria coadunata was collected from Panchakanya jungle, Dharan, Sunsari district. The collected ferns were shade dried, grind into powder and extracted with Methanol, Petroleum ether and aqueous extraction using Soxhlet extractor. The different solvents extract was evaluated and presence of phytochemicals like phenol, steroid, carbohydrates, glycosides, amino acid, alkaloids, flavonoids, terpenoids, saponins, and tannins. The TPC and TFC were also evaluated that ranged from 16.30 mg GAE/g to 57.48 mg GAE/g and 29.86 mg QE/g to 96.90 mg QE/g. Also tested for antimicrobial activity for two gram negative (Escherichia coli and Salmonella typhi) and two gram positive bacteria (Bacillus subtilis and Staphylococcus aureus). The antibacterial activity was done through disc diffusion method and Zone of inhibition (ZOI) was observed. The ZOI range from 8.5 to 23.5 mm. Overall result shows that the plant extracts have interesting antibacterial activity.