PHYTO CONSTITUENTS AND THEIR ANTIOXIDANT ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF FRUITS AND BARK OF TIMUR

Abstract

This study was aimed to assess the phyto constituents and antioxidant activity of fruit and bark of boke and sil timur, which were collected from village of Dhankuta. These sample were milled to fine powder and extracted with soxhlet apparatus in three different solvents. Then extracts were concentrated in rotary vacuum evaporator at 65°C under reduced pressure and stored at 2-8°C until analysis. The analysis was made for total phenol, total flavonoid, total tannin and DPPH free radical scavenging activity. From analysis it was found that, the total phenol content was found highest in boke timur (fruit) in methanolic extract (175.06 mg GAE/g), whereas lowest was found in sil timur (bark) in petroleum ether extract (19.33 mg GAE/g). The total flavonoid content was found highest in boke timur (fruit) in methanolic extract (91.42 mg QE/g), whereas lowest was found in boke timur (bark) in petroleum ether extract (10.29 mg QE/g). The total tannin content was found highest in sil timur (fruit) in methanolic extract (44.04 mg TAE/g), whereas lowest was found in sil timur (bark) in methanolic extract (6.46 mg TAE/g) and tannin was not detected in sil timur (bark) in ethanolic and petroleum ether extract. The DPPH radical scavenging activity was found highest in boke timur (fruit) in methanolic extract (IC50 =47.64 μg/mL), whereas lowest was found in sil timur (bark) in petroleum ether extract (IC50 =210.06 μg/mL. So extraction of phytochemical and antioxidant can be seen high in methanol and in boke timur (fruit).