Abstract:
The present study was carried out for microbiological analysis of raw meat marketed in Dharan Sub metropolitan city. Antibiotic resistance and its dissemination in food is a serious public health issue. The objective of study was to explore the occurrence and distribution of E. coli in meat and their susceptibility to antibiotics. Total of 24 samples (6 chicken, 6 buffalo,6 pork and 6 goats) were randomly collected when examined for stated microbiological parameters showed 41.66% Samples viz. 10 samples (3 Chicken, 3 Buffalo, 2 Pork, 2 Goat meat samples),were sorbitol non-fermenting E. coli. The highest Percentage of Sorbitol Non-Fermenting E. coli was found in Chicken and Buffalo meat with 50%, where’s as Pig and goat meat had the lowest prevalence with 33.33% occurrence.
All the isolated E. coli were subjected to the antibiotic susceptibility test using 17 different antibiotics namely Amoxicillin, Ampicillin, Ceftazidime, Cefixime, Amikacin, Ciprofloxacin, Chloramphenicol, Nalidixic acid, Erythromycin, Nitrofurantoin, Teicoplanin, Impenin, Cotrimoxzole, Gentamycin, Ceftriaxone, Cefotaxime and Tetracycline. All E. coli showed resistance (100%) against Ampicillin, Amoxicillin, Ceftazidime, and Teicoplanin. Out of 6 chicken C2 was found as the most pathogenic one because it showed resistance with greater number of drugs. The raw meat samples were found highly contaminated with sorbitol non-fermenting E. coli. And the people of Dharan are found at higher risk to be victim of such pathogenic E. coli. So, Strict hygienic practices, regular checks, sanitation, training to meat handlers, implementation of slaughterhouse and meat act and raising public awareness should be promoted to ensure quality of raw meat.
Description:
A
Project work report submitted to
Department of Microbiology
Central Campus of Technology, Tribhuvan University
In Partial Fulfillment for the Award of the Degree of
Bachelor of Science in Microbiology
