Abstract:
This study was conducted for the screening of oral flora and their antibiotic
susceptibility. The conventional culture method was employed in this study. 50
saliva sample were collected and proceeded first by mixing with Snyder agar tube
to observe that whether the organism responsible for tooth decay was present or
not. The pure culture was obtained from the subculture on Nutrient agar medium.
The isolates were subjected to gram staining and biochemical test. From the
biochemical test results, 5 isolated organisms were identified as Corynebacterium
and the remaining 45 organisms were identified as Neisseria spp.
The antibiotic sensitivity test of the isolated bacteria was performed using Mueller
Hinton Agar by disc diffusion method. From this test it was observed that
ampicillin and amoxicillin were mostly resistance to Corynebacterium ssp and
Neisseria spp while ciprofloxacin and erythromycin were sensitive to
Corynebacterium ssp and Neisseria spp.
Description:
Department of microbiology, CCT, Tribhuvan University Hattisar, Dharan November, 2016
