SCREENING OF ORAL FLORA AND THEIR ANTIBIOTIC SUSCEPTIBILITY

Abstract

This study was conducted for the screening of oral flora and their antibiotic susceptibility. The conventional culture method was employed in this study. 50 saliva sample were collected and proceeded first by mixing with Snyder agar tube to observe that whether the organism responsible for tooth decay was present or not. The pure culture was obtained from the subculture on Nutrient agar medium. The isolates were subjected to gram staining and biochemical test. From the biochemical test results, 5 isolated organisms were identified as Corynebacterium and the remaining 45 organisms were identified as Neisseria spp. The antibiotic sensitivity test of the isolated bacteria was performed using Mueller Hinton Agar by disc diffusion method. From this test it was observed that ampicillin and amoxicillin were mostly resistance to Corynebacterium ssp and Neisseria spp while ciprofloxacin and erythromycin were sensitive to Corynebacterium ssp and Neisseria spp.