Abstract:
The most frequent human gut pathogens are E. coli strains. The majority of
known E. coli strains are an etiologic cause of diarrhea in developing nations.
The spread of enteric pathogens into flowing water is a significant issue linked
with the disposal of these wastes. The study aimed to determine and
characterize the diarrheagenic E. coli isolated from river water of terai belt of
Province no.1. Altogether 43 river water sample were collected aseptically and
transported to the laboratory, then the collected sample were enriched and
cultured in EMB agar plate and incubated for 24 hrs at 37ºC. Furthermore,
gram staining and biochemical test were performed for the colony having
greenish metallic sheen. 18 positive isolated strains were subjected to
antibiotic susceptibility tests, biofilm assays, MIC. The data were statistically
analyzed with the help of SPSS version 25.
From the study, the overall prevalence of E. coli was 41.86%. The sensitivity
of E. coli isolates showed a significant difference in Amikacin, Cefotaxime,
Nalidixic acid, and Chloramphenicol whereas Ciprofloxacin was most
susceptible. Moreover, 72.22% were weak biofilm producers, followed by
moderate biofilm producers i.e., 5.56%, and 22.22% of isolates were non biofilm producers. According to the study, 44.44% of E. coli isolates were
sensitive to ciprofloxacin while 22.22% were resistant, 83.33% of isolates
were resistant to Ampicillin while 11.11% were sensitive. The 7-gene
multiplex PCR assays indicated that 0.14% (1/7) of isolates were harboring the
stx2 gene and 57.14% (4/7) of isolates were harboring escV and invE genes.
Virulent gene ent and pic was present in 14.28% (1/7) isolates. Atypical bfpB
and bla AmpC gene harboring were detected in 42.85(3/7) isolates
Description:
A Dissertation Submitted to the Department Of Microbiology,
CENTRAL CAMPUS OF TECHNOLOGY, Tribhuvan University,
Dharan, Nepal, in Partial Fulfillment of the Requirements for the
Award of Degree of Master of Science in Microbiology
(Public Health )
