Abstract:
Amylases are the enzymes that catalyzes the hydrolysis of starch, glycogen, and related polysaccharides to oligosaccharides, maltose, or glucose. In present study, amylase producing bacteria were isolated from five soil samples of different places of Dharan. A total of 25 bacterial colonies were isolated from collected soil samples. Five bacterial isolates, exhibited zone of clearance in starch hydrolysis test. The isolated displaying maximum amylase activity on quantitation were selected characteristics features of strain indicates that it belongs to the genus Bacillus will be later used for further characterization and amylase extraction. Maximum yield of amylase was obtained after 48hours incubation. The optimum pH for enzyme activity was found to be at pH 7.0 and the optimum temperature for the activity was found to be 40oC.
Description:
A
Project Work Submitted to
Department of Microbiology
Central Campus of Technology, Tribhuvan University
In Partial Fulfillment for the Award of the Degree of
Bachelor of Science in Microbiology
Submitted by
Chetan Maskey
Roll Number: 80015
Reg. Number: 5-2-0008-0029-2013
© Tribhuvan University
August, 2017
