Abstract:
This study aims to evaluate phytochemicals and antimicrobial activity of medicinal ferns
Tectaria coadunata. It was conducted from September 2022 to April 2023. Ferns which
have been commonly used by local people for medicinal purpose Tectaria coadunata was
collected from Panchakanya jungle, Dharan, Sunsari district. The collected ferns were
shade dried, grind into powder and extracted with Methanol, Petroleum ether and aqueous
extraction using Soxhlet extractor. The different solvents extract was evaluated and
presence of phytochemicals like phenol, steroid, carbohydrates, glycosides, amino acid,
alkaloids, flavonoids, terpenoids, saponins, and tannins. The TPC and TFC were also
evaluated that ranged from 16.30 mg GAE/g to 57.48 mg GAE/g and 29.86 mg QE/g to
96.90 mg QE/g. Also tested for antimicrobial activity for two gram negative (Escherichia
coli and Salmonella typhi) and two gram positive bacteria (Bacillus subtilis and
Staphylococcus aureus). The antibacterial activity was done through disc diffusion method
and Zone of inhibition (ZOI) was observed. The ZOI range from 8.5 to 23.5 mm. Overall
result shows that the plant extracts have interesting antibacterial activity.
